Journal: Molecular Biology of the Cell

Article Title: Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function

doi: 10.1091/mbc.E11-07-0658

Figure Lengend Snippet: RSK1 phosphorylation by PDK1 is required for its nuclear translocation. (A) Schematic of phosphorylation sites on rat RSK1. (B) Top two rows, serum-starved HeLa cells were treated with and without EGF (100 nM) for 10 min and the localization of total RSK1 was monitored with anti-RSK1 antibody; 4′,6-diamidino-2-phenylindole staining shows nuclei. Bottom four rows, the PDK1 inhibitor BX795 inhibits nuclear translocation of RSK1. HeLa cells were treated as described above with and without overnight preincubation with 0.5 μM BX795. The localization of active RSK1 was monitored with anti–phospho-T573-RSK antibody. (C) BX795 inhibits phosphorylation of RSK1 on S221 and S732. Cells were treated as in B. After immunoprecipitation with anti-RSK1 antibody, Western analyses of the immune complexes were performed with anti–phospho RSK antibodies. Scale bar, 10 μm. Representative data from three independent experiments are shown.

Article Snippet: Anti–phospho-RSK1/2 (S221) from R&D Systems (Minneapolis, MN), anti–phospho-RSK (T359/S363) from Cell Signaling (Beverly, MA), anti–phospho-RSK (S380) from Epitomics (Burlingame, CA), anti–phospho-RSK (T573) from Cell Signaling, and anti–phospho-RSK S732 raised by Rockland (Gilbertsville, PA) were used.

Techniques: Phospho-proteomics, Translocation Assay, Staining, Immunoprecipitation, Western Blot

Journal: Scientific Reports

Article Title: Integrative bioinformatics and molecular analysis revealed the roles of mTOR/S6K Axis, CASC15 , and miR-30a-3p in laryngeal squamous cell carcinoma

doi: 10.1038/s41598-026-39618-w

Figure Lengend Snippet: Genes/miRNAs/lncRNAs network. The red arrow indicates the direction of the mTOR/S6K axis. Green nodes denote common miRNAs that target mTOR and S6K . The lncRNAs linked to miR-30a-3p . Nodes represent miRNAs and lncRNAs associated with the mTOR/S6K axis. The edges represent predicted regulatory interactions based on TCGA, GEO, and other databases.

Article Snippet: The primary antibodies included mTOR (Cat No: 2971 S, Cell Signaling, 1:1000), S6K (Cat No: M01475-1, Boster Bio, 1:1000), and GAPDH (Cat No: ab8245, Abcam, 1:2500).

Techniques:

Journal: Scientific Reports

Article Title: Integrative bioinformatics and molecular analysis revealed the roles of mTOR/S6K Axis, CASC15 , and miR-30a-3p in laryngeal squamous cell carcinoma

doi: 10.1038/s41598-026-39618-w

Figure Lengend Snippet: The gene expression levels of mTOR , S6K , lncRNA CASC15 , and miR-30a-3p . mTOR (A), S6K (B), and CASC15 (C) genes are upregulated in LSCC tissues as compared to non-tumor tissues. miR-30a-3p (D) expression decreased in LSCC tissues. All data are expressed as mean ± SEM. (* p < 0.05 and **** p < 0.0001).

Article Snippet: The primary antibodies included mTOR (Cat No: 2971 S, Cell Signaling, 1:1000), S6K (Cat No: M01475-1, Boster Bio, 1:1000), and GAPDH (Cat No: ab8245, Abcam, 1:2500).

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Integrative bioinformatics and molecular analysis revealed the roles of mTOR/S6K Axis, CASC15 , and miR-30a-3p in laryngeal squamous cell carcinoma

doi: 10.1038/s41598-026-39618-w

Figure Lengend Snippet: The protein expression levels of mTOR and S6K in the study population. Western blot image, N: normal tissues; T: Tumor ( A ). The mTOR ( B ) and S6K ( C ) protein levels are upregulated in LSCC tissues compared with non-tumor tissues. All data are expressed as mean ± SEM. (**** P < 0.0001).

Article Snippet: The primary antibodies included mTOR (Cat No: 2971 S, Cell Signaling, 1:1000), S6K (Cat No: M01475-1, Boster Bio, 1:1000), and GAPDH (Cat No: ab8245, Abcam, 1:2500).

Techniques: Expressing, Western Blot